Motivation: Two human transcripts, FLJ11029 (AffyU133B: 228273_at) and C6orf173 (Affy U133b: 226936_at) has been previously associated with aggressive behavior of the breast carcinoma. We started with cloning of the full-size open reading frames of these transcripts into eukaryotic expression vectors capable of the support of both transient and stable expression in the human breast carcinoma cell lines. HCC2157 (Suspension Breast Primary Ductal Carcinoma Stage IIIA, grade II with doubling time of 2-3 days), MCF10A (non-tumorigenic Adherent Breast Fibrocystic Disease cell line with doubling time of 5-7 days) and NM2C5 (Adherent Breast Ductal Carcinoma with doubling time of 2-3 days).

Results: To be reported.